An efficient procedure of in vitro plant regeneration through direct shoot bud induction was tested for different explants of Capsicum annuum L. The best performance was observed for cotyledons on MS medium containing 6 mg/l 6- benzylaminopurine and 2 mg/l indole-3-butyric acid. Regeneration for other types of the explant (i.e. shoot-tip, hypocotyl and root) did not show satisfactory results because the explants did not develop into normal shoots but instead developed into calli after 12 days of culture. Histochemical analysis showed that only the cotyledons revealed a direct induction of more teratological protuberances that arise around the cut end of the explants. Elongation of shoot buds was obtained on MS medium containing 1 mg/l BAP + 0.5 mg/l IBA. Regenerated shoots rooted best on the same medium. After hardening, the rooted plants were transferred to the greenhouse condition where they normally grew, matured and flowered with a survival rate of 85%. We concluded that the present protocol can be efficiently used for mass propagation of sweet pepper.
کلید واژگان :Capsicum annuum L., Mass propagation, Micro propagation, Direct organogenesis, Explants, Regeneration, Plant growth regulators
ارزش ریالی : 300000 ریال
با پرداخت الکترونیک