Study of Peroxisome Proliferator-Activated Receptor gamma (PPAR-γ) of gastric mucosa in diabetics infected with Helicobacter Pylori

Introduction and objectives: Helicobacter Pylori Infection and diabetes mellitus are considered the major concerns and priorities of public health in modern and developed communities. The prevalence of H. Pylori infection has been reported increasing in diabetic patients. The aim of this study was to investigate peroxisome proliferator-activated receptor gamma (PPAR-GAMA), oxidative stress and nitrosative of stomach tissue in diabetics infected with H. Pylori in Tabriz city. Materials and Methods: In this study, three gastric antrum biopsy specimens, one gastric juice biopsy specimen and a total blood sample in vials containing the anticoagulant were taken form 60 diabetic patients infected with H. Pylori. As well, 60 non-diabetic patients uninfected with H. Pylori were sampled as a control group. One gastric biopsy specimen of the three was sent to a pathology laboratory for urease examination and another for pathological evaluation. The third biopsy was tested to measure total antioxidant capacity through total antioxidant assay kit made in England, and the PPAR-γ was measured by ELISA Kit (Chemiluminescence) manufactured by Signosis. Hemoglobin A1C (HbA1c) was assessed using the ion exchange chromatography method by commercial kit (Biosystem, Spain), and gastric juice of patients was used to measure the NO radical with Grace Colorimetric method. Results: The mean age of the study group was 39/52±12/95 and of the control group was 34/87±15/33 years, who were not statistically significantly different (p=0.15). A significant increase in PPAR- γ receiver mean in gastric mucosa cell nucleus was observed in the case group in comparison with that of the control group (p=0.007). The mean of nitric oxide (NO)in gastric juice in the control group was significantly different from that of the control group, being much lower than in the control group (p =0.0001).The percentage of glycated hemoglobin was significantly higher in the group than in the control group and showed a significant increase as the p-value was less than 0.0001. Gastric tissue total antioxidant capacity in the case group was significant from that of the control group, and the decreased level revealed that the p-value equaled to 0.012. Conclusions: The results showed that firstly, H. Pylori infection in diabetic people controls hyperglycemia very badly, and may cause the problems of blood glucose increase more complex in these patients. Secondly, the increase in oxidative stress and nitrosative, and also the increased level of nuclear PPAR- γ receptors in stomach tissue increase the risk of cancer in stomach tissue in diabetic patients infected with H. Pylori.

Helicobacter Pylori, diabetes mellitus, Peroxisome proliferator-activated receptor gamma (PPAR-GAMA), Oxidative and Nitrosative stresses