Objective: This study explored effects of LLLT on cellular changes in organ cultures of skin from streptozotocin–diabetic ( STZ-D) rats. Background Data: Skin and its fibroblasts are impaired in diabetes. Therefore, healing of skin wounds, are impaired in diabetic patients. The positive effects of low–level laser therapy (LLLT) on complications of diabetes in patients and animal models have been shown. Methods: Diabetes induced in rats by streptozotocin .After 30 days from STZ injection, two sets of skin samples were extracted from skin under sterile conditions. One set of samples were proliferated in vitro and another ones were cultured as organ culture. An 24 – well culture medium contained Dolbeccos modified minimum essential medium , and supplemented by 12% fetal bovine serum. There were five laser– treated and their relevant sham–exposed groups. A helium–neon laser was used. Low level laser with 0.9 – 4J/cm2 energy densities was applied four times to each organ and cell cultures. The cell cultures were analyzed by light microscopy and transmission electron microscopy examinations. Cell proliferations were evaluated by dimethyl thiazol-diphenyl terazolium bromide (MTT) assay. Results: Statistical analysis revealed that 4 J/cm2 irradiation significantly increases the cell numbers comparing to relevant sham – exposed cultures (p = 0.017).
کلید واژگان :Animal, Basic Science, Biology, Cell Culture, Biostimulation
ارزش ریالی : 600000 ریال
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