The temperature-induced, over-expression of the human growth hormone gene in a recombinant E. coli during
high cell density cultivation is reported. Human growth hormone (hGH) production and stability were tested under
different heat shock conditions. Cell densities were 25 and 60 g l−1 in a pH-stat fed-batch mode in defined and
complex medium, respectively, and the fermentation time was decreased from 41 to 32 h. hGH was produced at
2 g l−1 in complex medium. By using glycerol as main carbon source in the complex medium with exponential
feeding, cell density and hGH production were increased to 100 g l−1 and 2.7 g l−1, respectively.
کلید واژگان :
heat-inducible expression, high cell density cultivation, human growth hormone, recombinant Escherichia coli
