Wild common carp populations from southern Caspian Sea were examined for transferrin polymorphism, PCR-RFLP and microsatellite DNA variations. Transferrin variability (1-2) allele per locus and observed heterozygote was 0.1 at 3 loci that was lower than RFLP in which the average haplotype was 0.48 for ND-3/4 and ND-5/6 gene and much lower than microsatellite variability (5.5-13 alleles per locus, Expected heterozygocity 0.694-0.856 at five loci). There was very low variation in transferrin variations, but more pronounced at microsatellite loci. Genetic diversity showed that for microsatellite loci most of variation was due to the within population component. 13 and 9 different haplotype in ND-3/4 and ND-5/6 gene of common carp were detected Therefore by applying transferrin and mitochondrial marker could be well characterized generally, genetically and geographically and it could be used for breeding program as well as comparing the differences of races of quite different population, while the microsatellite are better suited to detect the detail structural genetic of population and loss of variation due to inbreeding and departure from Hardy-Weinberg Equilibrium.
کلید واژگان :Transferrin polymorphism % PCR-RFLP % Microsatellite DNA % Common carp
ارزش ریالی : 300000 ریال
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