Abstract: The objective of this study was fractionation of Lizard Leishmania promastigote. Mass culture of lizard Leishmania promastigote was provided. Fractionation was carried out by ammonium sulfate precipitation and size exclusion chromatography. The 19.2, 21.5, 25, 30, 35, 64 and 78 kDa fractions were found and they used for vaccination against mouse leishmaniasis.
