IgM-like immunoglobulins (Igs) were isolated from pooled anti-goat IgG sera raised in five
species of sturgeons consisting of great sturgeon (Huso huso), Russian sturgeon (Acipenser
guldenstedti), ship sturgeon (A. nudiventris), starred (stellate) sturgeon (A. stellatus) and Persian
sturgeon (A. persicus). The Igs were purified by affinity chromatography. The molecular weight
(MW) of the non-reduced IgMs from all species was identical with 870 kilo Daltons (kDa)
estimated by dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The MW of the
heavy and light chains was estimated at 77-84 and 28-30 kDa, respectively.
کلید واژگان :
polyacrylamide gel electrophoresis, Acipenser guldenstedti, IgM-like immunoglobulins
