Shrimp is one of the most important fishery products of the coastal provinces in the Persian Gulf in Iran. Vibriosis has been an important cause of production loss due to bacterial disease in shrimp farms in south Iran in recent years. The objective of this study was to detect the prevalence of Vibrio spp. in shrimp samples from farms in the southern provinces of Iran by polymerase chain reaction (PCR). A total number of 36 shrimp were caught from south coast of Iran and were studied to identify Vibrio spp. Three Vibrio species including Vibrio parahemolyticus, V. alginolyticus and V. harveyi were identified using biochemical and molecular methods. Two shrimp contained one or more Vibrio spp. as two samples contained V. parahemolyticus, two samples contained V. alginolyticus and two contained V. harveyi. The results of the present study revealed that the shrimp from the studied area is contaminated with Vibrio spp. and molecular detection systems were applied for the investigation of shrimp samples for the presence of the different Vibrio spp. The collected data indicate that the PCR systems can be useful for rapid detection and differentiation of Vibrio spp. in shrimp samples as basis for preventive protection policy to consumers.
کلید واژگان :prevalence, molecular method, vibriosis, shrimp, Iran
ارزش ریالی : 600000 ریال
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