An experiment was carried out to examine the effects of different combinations of plant growth regulators in vitro micropropagation of strawberry. Strawberry of the Rosaceae family, is a perennial, stoloniferous herb and popular in genetic engineering. The present study showed the procedure for propagation of Fragaria using nodal segments from in vitro germinated plants. The best concentration of BAP for bud induction was 0.5 mg/l plus KIN 0.2 mg/l. Histochemical analysis showed that only a direct induction of more teratological protuberances that arise around the cut end of the explants. The maximum number of roots per explant was obtained in elongation medium in MS combined with BAP 0.1 mg/l with auxin IBA 0.2 mg/l. In this medium, all of them the seedlings had safe and excellent rooting. The rooted plants were transferred to the greenhouse condition where they normally grew, matured and flowered with a survival rate of 100%. It is concluded that the present protocol can be efficiently used for mass propagation of strawberry.
کلید واژگان :Micropropagation, Fragaria, Nodal segments, Rosaceae Abbreviations: BAP―6-benzylaminopurine; IBA― indole-3- butyric acid; KIN: kinetin; AC: activated charcoal.
ارزش ریالی : 300000 ریال
با پرداخت الکترونیک