cause severe damages to the fetus including spontaneous abortion, stillbirth and neurological signs. Its severity is gestational time-dependent (1). According to previous studies, maternal infection in the first and second trimester
may be resulted in prenatal death or stillbirth in 5% and 2 % of the cases, respectively. The major diagnostic
assays for toxoplasmosis are serological methods despite
many limitations (3). Molecular method have been developed to improve the diagnosis of congenital toxoplasmosis
. Polymerase Chain Reaction (PCR)-based assay
to amplify a 200-300 fold reprtitive 529-base pair (bp) DNA region of Toxoplasma has a higher sensitivity than conventional serological tests . Development of an accurate,
sensitive and rapid method for the detection and identification of T. gondii is important in both diagnosis and treatment ).
