Two new isogenes encoding heavy metal ATPase have been partially cloned from Aeluropus littoralis genome. One of them was 849 bp in length and related to an isoform which encodes a copper-transporting P1B-ATPase (AlHMA1), and the other one was 403 bp in length and related to an isoform encoding a zinc-transporting P1B-ATPase (AlHMA2). When southern blot hybridization was performed under low-stringency, 6 to 7 hybridization bands appeared, indicating that in this plant heavy metal ATPase is encoded by a multigene family. RT-PCR assay displayed that the AlHMA2 highly expresses in leaf and root and its transcription level is the same in both organs. Phylogenetic tree showed that copper transporters from different plants were located in one group and zinc transporters were located in another group, although all heavy metal P -ATPases have the same function. It means the different 1Bisoforms in single plant that involve in transporting of different heavy metals, may come from different sources.
کلید واژگان :gene cloning, tissue expression pattern, Gouan, heavy metals ATPase, metal-transporting.
ارزش ریالی : 1200000 ریال
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