Prior to the production of human gamma interferon using recombinant DNA technology, it had been produced mainly upon mitogenic induction of lymphocytes in very low amounts, which evidently hampered its characterization and its medical applications. The recombinant gamma interferons produced in larger quantities in prokaryotic systems retain their biological activities, and can be used clinically in the treatment of various viral, neoplastic and immunosuppressed conditions or diseases. In this study, a cDNA sequence coding for human gamma interferon was synthesized from mRNA template extracted from induced human T lymphocytes. The cDNA was then amplified by PCR, cloned in an expression vector, and transformed into Escherichia coli. The polypeptide produced through the expression of this DNA sequence in E. coli showed immunological and chemical properties resembling authentic human IFN-γ.
کلید واژگان :Gamma interferon, complementary DNA, Polymerase chain reaction, cloning, expression, polyclonal antibodies, Immunoblotting
ارزش ریالی : 1200000 ریال
با پرداخت الکترونیک