Andrographis paniculata is an important medicinal herb in the family of the Acanthaceae. Low seed germination is one of the main problems in A. paniculata. The current study focused on the seed dormancy and effective methods to enhance the seed germination. For this purpose, two experiments were carried out: (1) Testing different mechanical and chemical scarification methods; (2) studying the impact of seed and seed coat proteins on the scarified seeds. The results showed that all scarification methods increased the germination percentage, while seed and seed coat protein extracts decreased it. The fastest and highest germination was obtained using scarification with sandpaper (71.33% at the third day after the scarification). The germination was further increased up to 94% after 20 days. Finally, to identify the role of seed and seed coat proteins on the seed dormancy and germination, the proteins were analyzed on the sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Three polymorphic bands were detected in total. However, the hard seed coat layer may physically affect the seed dormancy, but the presence of a specific protein sized 75 kDa in the seed coat and two proteins sized 31 and 69 kDa in endosperm may cause the physiological dormancy. Our findings suggested that a combination of physical and physiological mechanisms control the dormancy of A. paniculata seed.
کلید واژگان :Andrographis paniculata, seed protein, scarification, seed dormancy, seed germination
ارزش ریالی : 1200000 ریال
با پرداخت الکترونیک