Protective Role of Ethyl Pyruvate on Sperm Fertility of Mice Infected with Hemolytic Anemia Resulting from administrating Phenylhydrazine

Background and aims: The reports were showed that oxygen shortage is one of the harmful factors on testis especially the efficacy of sperms. This hypoxia can be made due to ailment conditions such as different kinds of anemia, poisonings, working in places with shortage of oxygen or living in high places. Since Phenylhydrazine (PHZ) is known as a proper substance in making of anemia condition, it is used in this study. In this survey, the aim is to investigate the protective role of Ethyl Pyruvate on the sperm fertilizing amount of animals concerned with hypoxia resulting from induced hemolytic anemia by PHZ and based on “In Vitro Fertilization” or IVF. Materials & Methods: 24 adult mice (weighing 25-20 gr at 6 to 8 weeks of age) were divided equally into 4 groups in that group 1, control group, that received normal saline (0.1ml, IP).The group 2 was received phenylhydrazine (PHZ) with dose of 8mg/100 gr b. w, IP at first time and followed by 6mg/100 gr b. w/48h, IP. The group 3 received ethyl pyruvate (40 mg/kg, IP) along with PHZ and the group 4 received only ethyl pyruvate (40 mg/kg, IP). After 35 days, the sperm samples were collected from the epididymis and were induced using IVF methods with healthy oocytes. Results: The results indicate the considerable reduction of fertilized oocytes, two-cell zygote as well as blastocyst, and the number of arrested zygotes in PHZ group. But ethyl pyruvate could improve the IVF results in some extent. Conclusion: These results suggested the protective effect of ethyl pyruvate on oxidative stress caused by hypoxia-induced hemolytic anemia with PHZ on the IVF of mice sperm.

Phenylhydrazine, Hemolytic anemia, Ethyl pyruvate, IVF